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Single‐Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions
Author(s) -
Novak Richard,
Zeng Yong,
Shuga Joe,
Venugopalan Gautham,
Fletcher Daniel A.,
Smith Martyn T.,
Mathies Richard A.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201006089
Subject(s) - multiplex , amplicon , primer (cosmetics) , computational biology , sorting , multiplex polymerase chain reaction , agarose , computer science , dna sequencing , gene , biology , genetics , microbiology and biotechnology , polymerase chain reaction , chemistry , programming language , organic chemistry
Solitary confinement : The described approach enabled high‐throughput DNA preparation followed by multiplex emulsion PCR for the detection and sequencing of genetic targets (see picture). No droplet sorting was required, and single‐cell segregation was maintained throughout owing to the incorporation of primer‐functionalized beads as amplicon substrates.