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Chemical Synthesis of Ubiquitin, Ubiquitin‐Based Probes, and Diubiquitin
Author(s) -
El Oualid Farid,
Merkx Remco,
Ekkebus Reggy,
Hameed Dharjath S.,
Smit Judith J.,
de Jong Annemieke,
Hilkmann Henk,
Sixma Titia K.,
Ovaa Huib
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201005995
Subject(s) - dipeptide , ubiquitin , chemistry , combinatorial chemistry , peptide , ubiquitin ligase , stereochemistry , biochemistry , gene
Changing the subject : An efficient linear solid‐phase peptide synthesis of ubiquitin (Ub) has been developed. This approach allows the incorporation of desired tags and mutations (see picture; blue denotes a pseudoproline dipeptide, red a dimethoxybenzyl dipeptide) as well as specific C‐terminal modification and the construction of all diubiquitin conjugates in high yields and purities in a straightforward manner.