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Fluorescence Imaging of Influenza H1N1 mRNA in Living Infected Cells Using Single‐Chromophore FIT‐PNA
Author(s) -
Kummer Susann,
Knoll Andrea,
Socher Elke,
Bethge Lucas,
Herrmann Andreas,
Seitz Oliver
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201005902
Subject(s) - chromophore , virus , messenger rna , orange (colour) , fluorescence , nucleic acid , virus inactivation , chemistry , computational biology , biology , virology , biochemistry , gene , food science , optics , physics , photochemistry
A message from the virus was detected by a peptide nucleic acid probe that contains thiazole orange as a fluorescent base surrogate (see picture). The high specificity and biostability of the probes lead to significant improvements to the signal‐to‐background ratio in imaging the mRNA from the influenza H1N1 virus in living infected cells.