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Self‐Immobilizing Fluorogenic Imaging Agents of Enzyme Activity
Author(s) -
Kwan David H.,
Chen HongMing,
Ratananikom Khakhanang,
Hancock Susan M.,
Watanabe Yoichiro,
Kongsaeree Prachumporn T.,
Samuels A. Lacey,
Withers Stephen G.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201005705
Subject(s) - enzyme , fluorescence , in vivo , cell sorting , enzyme assay , chemistry , sorting , biochemistry , computer science , staining , cell , information retrieval , combinatorial chemistry , computational biology , biology , microbiology and biotechnology , genetics , quantum mechanics , physics , programming language
Lighting up enzyme activity : Fluorogenic enzyme substrates have been developed that upon hydrolysis form reactive quinone methides, which can react with cell components in vivo to generate an immobilized fluorescent label (see scheme). These compounds can be used for the histological staining of tissues or activity‐based screening of cells by fluorescence‐activated cell sorting.