Premium
Insights into the Finer Issues of Native Chemical Ligation: An Approach to Cascade Ligations
Author(s) -
Tan Zhongping,
Shang Shiying,
Danishefsky Samuel J.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201005513
Subject(s) - ligation , computer science , diastereomer , protocol (science) , cascade , native chemical ligation , information retrieval , computational biology , chemistry , biology , stereochemistry , biochemistry , chemical synthesis , microbiology and biotechnology , medicine , chromatography , alternative medicine , pathology , in vitro
Maximizing by minimizing : An efficient and broadly useful two‐step ligation protocol was developed. Important mechanistic issues of ligation were probed from competition studies on the formation of diastereomeric ligation products. This study provides a valuable approach to facilitate polypeptide synthesis by minimizing protecting group manipulations and intermediate isolations.