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Pyrene‐Excimer Probes Based on the Hybridization Chain Reaction for the Detection of Nucleic Acids in Complex Biological Fluids
Author(s) -
Huang Jin,
Wu Yanrong,
Chen Yan,
Zhu Zhi,
Yang Xiaohai,
Yang Chaoyong James,
Wang Kemin,
Tan Weihong
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201005375
Subject(s) - pyrene , nucleic acid , excimer , chemistry , chain reaction , fluorescence , dna , nucleic acid thermodynamics , molecular probe , combinatorial chemistry , photochemistry , base sequence , organic chemistry , biochemistry , physics , quantum mechanics
Make it a double : Complementary probes with two pyrene labels were engineered for the amplification of a target DNA sequence. In the stem‐closed conformation of the probes in the absence of the target, the two pyrene moieties were separated. The target propagated hybridization chain reactions to bring pyrene moieties on neighboring probes close together to form fluorescent pyrene excimers (see picture).