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RNA‐Directed Packaging of Enzymes within Virus‐like Particles
Author(s) -
Fiedler Jason D.,
Brown Steven D.,
Lau Jolene L.,
Finn M. G.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201005243
Subject(s) - rna , enzyme , computational biology , virus , aptamer , computer science , biochemistry , chemistry , biology , microbiology and biotechnology , virology , gene
Packaged molecular machines are available using dual expression vectors that guide the preparation of Qβ virus‐like particles encapsulating multiple enzymes. Packaging is promoted by RNA aptamer sequences that bridge between the coat protein and a peptide tag fused to the desired cargo (see scheme). Peptidase E and luciferase were thus encapsulated and shown to be catalytically active inside the particle.