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Modulating PNA/DNA Hybridization by Light
Author(s) -
Stafforst Thorsten,
Hilvert Donald
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201004548
Subject(s) - photoswitch , azobenzene , peptide nucleic acid , dna , transcription (linguistics) , chemistry , nucleic acid , rna , computational biology , microbiology and biotechnology , biology , biochemistry , gene , linguistics , molecule , philosophy , photochemistry , organic chemistry
Turn it on! Hybridization of peptide nucleic acids with DNA can be efficiently manipulated by modifying the PNA with a single azobenzene photoswitch. The effect is remarkably strong in the triplex binding mode and can be exploited for the photocontrol of transcription by T7 RNA polymerase (Pol T7).