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Selective Detection of 5‐Formyl‐2′‐deoxyuridine, an Oxidative Lesion of Thymidine, in DNA by a Fluorogenic Reagent
Author(s) -
Hirose Wataru,
Sato Kousuke,
Matsuda Akira
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201004087
Subject(s) - thymidine , derivatization , reagent , dna , chemistry , deoxyuridine , lesion , fluorescence , biochemistry , combinatorial chemistry , chromatography , high performance liquid chromatography , microbiology and biotechnology , biology , organic chemistry , medicine , physics , quantum mechanics , psychiatry
Chemoselectivity in DNA : The thymidine lesion 5‐formyl‐2′‐deoxyuridine ( 1 ) induces the mutation of DNA. Its derivatization with the specific fluorogenic reagent 2‐amino‐4,5‐dimethoxythiophenol ( 2 ) gives 3 , which shows strong fluorescence. This fast method for the detection of 1 requires no enzymatic digestion, HPLC separation, or MS analysis. ROS=reactive oxygen species.