Selective Detection of 5‐Formyl‐2′‐deoxyuridine, an Oxidative Lesion of Thymidine, in DNA by a Fluorogenic Reagent | Zendy

Hirose Wataru | Zendy; Sato Kousuke | Zendy; Matsuda Akira | Zendy

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Selective Detection of 5‐Formyl‐2′‐deoxyuridine, an Oxidative Lesion of Thymidine, in DNA by a Fluorogenic Reagent

Author(s) -

Hirose Wataru,

Sato Kousuke,

Matsuda Akira

Publication year - 2010

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201004087

Subject(s) - thymidine , derivatization , reagent , dna , chemistry , deoxyuridine , lesion , fluorescence , biochemistry , combinatorial chemistry , chromatography , high performance liquid chromatography , microbiology and biotechnology , biology , organic chemistry , medicine , physics , quantum mechanics , psychiatry

Chemoselectivity in DNA : The thymidine lesion 5‐formyl‐2′‐deoxyuridine ( 1 ) induces the mutation of DNA. Its derivatization with the specific fluorogenic reagent 2‐amino‐4,5‐dimethoxythiophenol ( 2 ) gives 3 , which shows strong fluorescence. This fast method for the detection of 1 requires no enzymatic digestion, HPLC separation, or MS analysis. ROS=reactive oxygen species.

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