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A Highly Efficient Strategy for Modification of Proteins at the C Terminus
Author(s) -
Yi Long,
Sun Hongyan,
Wu YaoWen,
Triola Gemma,
Waldmann Herbert,
Goody Roger S.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201003834
Subject(s) - posttranslational modification , n terminus , c terminus , computational biology , chemistry , computer science , biochemistry , biology , peptide sequence , amino acid , enzyme , gene
A new linker : In a facile, chemoselective, and potentially general method for protein modification at the C terminus oxyamino‐modified proteins obtained from protein thioesters react rapidly with ketones under mild conditions. This strategy was used for fluorescence labeling, for example with modified coumarin and fluorescein (see scheme).