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Small‐Molecule Sensing: A Direct Enzyme‐Linked Immunosorbent Assay for the Monosaccharide Kdo
Author(s) -
Mannerstedt Karin,
Jansson Anita M.,
Weadge Jody,
Hindsgaul Ole
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201003435
Subject(s) - adduct , monosaccharide , enzyme , small molecule , covalent bond , chemistry , molecule , stereochemistry , combinatorial chemistry , biochemistry , organic chemistry
Captured and bound! Kdo, a monosaccharide, and an immobilized capture molecule form a covalent adduct that can be detected by adduct‐specific antibodies (see scheme). The recognition yields a signal that is proportional to the amount of Kdo present in solution. This small‐molecule sensor should be applicable for any small molecule that can react to give an immunogenic adduct.