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Efficient Access to Nonhydrolyzable Initiator tRNA Based on the Synthesis of 3′‐Azido‐3′‐Deoxyadenosine RNA
Author(s) -
Steger Jessica,
Graber Dagmar,
Moroder Holger,
Geiermann AnnaSkrollan,
Aigner Michaela,
Micura Ronald
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201003424
Subject(s) - transfer rna , chemistry , deoxyadenosine , amide , combinatorial chemistry , hydrolysis , nucleic acid , rna , stereochemistry , biochemistry , adenosine , gene
Flexibility exercised : Hydrolysis‐resistant 3′‐aminoacyl‐tRNA conjugates that contain a stable amide linkage instead of the natural ester are valuable substrates for biochemical studies of ribosomal processes. In a novel preparation of the stable E. coli initiator tRNA derivative 3′‐( N ‐formylmethionyl)amino‐tRNA fMet the key feature is the synthesis of 3′‐azido oligoribonucleotides using a new functionalized solid support.