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cat‐ELCCA: A Robust Method To Monitor the Fatty Acid Acyltransferase Activity of Ghrelin O ‐Acyltransferase (GOAT)
Author(s) -
Garner Amanda L.,
Janda Kim D.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201003387
Subject(s) - acyltransferase , ghrelin , enzyme , click chemistry , chemistry , nat , analyte , biochemistry , computational biology , combinatorial chemistry , computer science , biology , chromatography , hormone , computer network
Assays armed with catalytic signal amplification have arisen as superior systems for ultrasensitive detection of analytes. A conceptually new enzyme assay called cat‐ELCCA (catalytic assay using enzyme‐linked click‐chemistry) is described, in which an enzyme‐linked azide is utilized to arm the assay with catalytic fluorescence signal amplification. By using this assay technology, the first high‐throughput screen for recently disclosed ghrelin O ‐acyltransferase (GOAT) was developed.