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Microarray‐Assisted High‐Throughput Identification of a Cell‐Permeable Small‐Molecule Binder of 14 ‐ 3 ‐ 3 Proteins
Author(s) -
Wu Hao,
Ge Jingyan,
Yao Shao Q.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201003257
Subject(s) - small molecule , identification (biology) , computational biology , computer science , in vitro , microarray , information retrieval , chemistry , microbiology and biotechnology , combinatorial chemistry , world wide web , biology , gene , biochemistry , gene expression , botany
The way to a 14‐3‐3 binder : A fragment‐based combinatorial small‐molecule microarray generates affinity‐based fingerprints of the 14‐3‐3σ protein. One small molecule (see picture; in red box) that disrupts the 14‐3‐3/protein interaction (green/blue) has been identified. The compound is cell‐permeable and possesses both in vitro and in‐cell activities.