Precise Control of Protein Concentration in Living Cells | Zendy

Lau Hubert D. | Zendy; Yaegashi Junko | Zendy; Zaro Balyn W. | Zendy; Pratt Matthew R. | Zendy

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Precise Control of Protein Concentration in Living Cells

Author(s) -

Lau Hubert D.,

Yaegashi Junko,

Zaro Balyn W.,

Pratt Matthew R.

Publication year - 2010

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201003073

Subject(s) - ubiquitin , complementation , chemistry , microbiology and biotechnology , protein degradation , degradation (telecommunications) , biophysics , biology , biochemistry , computer science , mutant , gene , telecommunications

Rapamycin to the rescue : Native protein concentration was precisely controlled in living cells by using the small molecule rapamycin. The addition of rapamycin “shielded” a destabilization domain from degradation and induced complementation of split ubiquitin (UbN and UbC); as a result, proteins (Pro) of interest were released. This technology enables the investigation of native protein biology over a broad concentration range.

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