Premium
Amplified Aptamer‐Based Assay through Catalytic Recycling of the Analyte
Author(s) -
Lu ChunHua,
Li Juan,
Lin MeiHua,
Wang YiWei,
Yang HuangHao,
Chen Xi,
Chen GuoNan
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201002822
Subject(s) - aptamer , nuclease , analyte , nanomaterials , deoxyribozyme , chemistry , dna , cleavage (geology) , nanotechnology , combinatorial chemistry , chromatography , microbiology and biotechnology , materials science , biology , biochemistry , fracture (geology) , composite material
Turn up the volume : In a simple and highly sensitive aptamer‐based assay that relies on the ability of nanomaterials to protect DNA from nuclease cleavage, the aptamer is released from the nanomaterial when challenged with a target. The nuclease then cleaves the free aptamer and releases the target, which can initiate a new cycle. Recycling of the target in this way leads to significant signal amplification (see diagram).
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom