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Amplified Aptamer‐Based Assay through Catalytic Recycling of the Analyte
Author(s) -
Lu ChunHua,
Li Juan,
Lin MeiHua,
Wang YiWei,
Yang HuangHao,
Chen Xi,
Chen GuoNan
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201002822
Subject(s) - aptamer , nuclease , analyte , nanomaterials , deoxyribozyme , chemistry , dna , cleavage (geology) , nanotechnology , combinatorial chemistry , chromatography , microbiology and biotechnology , materials science , biology , biochemistry , fracture (geology) , composite material
Turn up the volume : In a simple and highly sensitive aptamer‐based assay that relies on the ability of nanomaterials to protect DNA from nuclease cleavage, the aptamer is released from the nanomaterial when challenged with a target. The nuclease then cleaves the free aptamer and releases the target, which can initiate a new cycle. Recycling of the target in this way leads to significant signal amplification (see diagram).