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Cellular Integration of an Enzyme‐Loaded Polymersome Nanoreactor
Author(s) -
van Dongen Stijn F. M.,
Verdurmen Wouter P. R.,
Peters Ruud J. R. W.,
Nolte Roeland J. M.,
Brock Roland,
van Hest Jan C. M.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201002655
Subject(s) - polymersome , nanoreactor , pinocytosis , horseradish peroxidase , intracellular , chemistry , nanotechnology , enzyme , microbiology and biotechnology , cell , biophysics , biochemistry , amphiphile , biology , materials science , nanoparticle , copolymer , endocytosis , organic chemistry , polymer
Cells with implants: Porous enzyme‐loaded polymersomes were constructed that display the cell‐penetrating peptide tat on their surfaces. These nanoreactors are taken up by mammalian cells through macropinocytosis. Inside the cells, the polymersomes are only partially routed to acidic compartments. Polymersomes with horseradish peroxidase as a model cargo enzyme displayed sustained intracellular activity.