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A Significant Improvement of the Efficacy of Radical Oxidant Probes by the Kinetic Isotope Effect
Author(s) -
Kundu Kousik,
Knight Sarah F.,
Lee Seungjun,
Taylor W. Robert,
Murthy Niren
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201002228
Subject(s) - deuterium , kinetic isotope effect , chemistry , in vivo , superoxide , isotope , hydroxyl radical , combinatorial chemistry , radical , photochemistry , organic chemistry , biology , nuclear physics , physics , microbiology and biotechnology , enzyme
A breath of fresh air : The rate of aerial oxidation of dihydroethidium and hydrocyanine radical oxidant probes can be selectively reduced by deuteration (see picture). The reaction rate between the deuterated compounds and the superoxide radical was reduced by a much smaller factor because of mechanistic differences between the two reactions. The deuterated probes are more effective than their hydrogen analogues in vitro, in cell culture, and in vivo.