Premium
Orthogonal Alkynyl Amino Acid Reporter for Selective Labeling of Bacterial Proteomes during Infection
Author(s) -
Grammel Markus,
Zhang Mingzi M.,
Hang Howard C.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201002050
Subject(s) - haystack , salmonella , proteome , amino acid , chemistry , computational biology , bacteria , biology , combinatorial chemistry , biochemistry , computer science , world wide web , genetics
Bacterial proteins in the haystack : The alkynyl amino acid reporter AOA (see scheme) can be used to visualize and identify newly synthesized bacterial proteins during Salmonella infection of mammalian cells by the copper(I)‐catalyzed azide–alkyne cycloaddition. This technique opens new opportunities for the system‐wide analysis of bacterial pathogens.