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Quantification of the Sixth DNA Base Hydroxymethylcytosine in the Brain
Author(s) -
Münzel Martin,
Globisch Daniel,
Brückl Tobias,
Wagner Mirko,
Welzmiller Veronika,
Michalakis Stylianos,
Müller Markus,
Biel Martin,
Carell Thomas
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201002033
Subject(s) - 5 hydroxymethylcytosine , dna , nucleoside , base (topology) , base pair , biology , computational biology , chemistry , dna methylation , genetics , mathematics , gene , mathematical analysis , gene expression
Mind over matter : LC‐MS has allowed the amount of the post‐replicatively formed DNA base 5‐hydroxymethylcytosine (see picture; left) to be quantified in brain tissue. The nucleoside is most abundant in areas that are associated with higher cognitive functions, and its content in mouse hippocampi seems to increase with age. The new method enables hydroxymethylcytosine to be quantified with unprecedented accuracy.