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A Cleavable Linker Based on the Levulinoyl Ester for Activity‐Based Protein Profiling
Author(s) -
Geurink Paul P.,
Florea Bogdan I.,
Li Nan,
Witte Martin D.,
Verasdonck Joeri,
Kuo ChiLin,
van der Marel Gijs A.,
Overkleeft Herman S.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001767
Subject(s) - linker , proteasome , profiling (computer programming) , chemistry , combinatorial chemistry , biochemistry , computer science , operating system
Get linked : The title linker is stable under various biological conditions and can be cleaved chemoselectively with hydrazine (see scheme). Its use is demonstrated in the activity‐based enrichment and identification of proteasome active subunits from cell extracts.