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Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering
Author(s) -
Prokop Zbynek,
Sato Yukari,
Brezovsky Jan,
Mozga Tomas,
Chaloupkova Radka,
Koudelakova Tana,
Jerabek Petr,
Stepankova Veronika,
Natsume Ryo,
van Leeuwen Jan G. E.,
Janssen Dick B.,
Florian Jan,
Nagata Yuji,
Senda Toshiya,
Damborsky Jiri
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001753
Subject(s) - loop (graph theory) , selectivity , chemistry , directed evolution , point mutation , feedback loop , stereochemistry , combinatorial chemistry , computer science , mutation , gene , combinatorics , mathematics , organic chemistry , biochemistry , world wide web , mutant , catalysis
In the loop : Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (ln E versus 1/ T ) of β‐bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single‐point mutation is made. This behavior is not observed for α‐bromoesters.