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Posttranscriptional Signal Integration of Engineered Riboswitches Yields Band‐Pass Output
Author(s) -
Muranaka Norihito,
Yokobayashi Yohei
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001482
Subject(s) - riboswitch , signal (programming language) , synthetic biology , simple (philosophy) , green fluorescent protein , complementation , escherichia coli , computer science , chemistry , biology , rna , computational biology , biochemistry , gene , phenotype , philosophy , epistemology , programming language , non coding rna
Light in the middle : A simple band‐pass circuit has been designed using only posttranscriptional components in Escherichia coli (see figure). Two engineered riboswitches were used as the chemical sensor and to implement low‐pass/high‐pass functions. The two signals were integrated by protein complementation using split GFP fragments to achieve a band‐pass response.