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Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In‐Stem Molecular Beacon
Author(s) -
Hara Yuichi,
Fujii Taiga,
Kashida Hiromu,
Sekiguchi Koji,
Liang Xingguo,
Niwa Kosuke,
Takase Tomokazu,
Yoshida Yasuko,
Asanuma Hiroyuki
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001459
Subject(s) - fluorophore , quenching (fluorescence) , molecular beacon , scheme (mathematics) , computer science , chemistry , rational design , combinatorial chemistry , fluorescence , photochemistry , information retrieval , materials science , physics , nanotechnology , optics , mathematics , biochemistry , dna , mathematical analysis , oligonucleotide
Excitonic interaction was utilized to design a highly sensitive in‐stem molecular beacon (ISMB) in which both a fluorophore and a quencher on D ‐threoninols are incorporated as a pseudo base pair (see scheme; optimized combination with Cy3 and modified Methyl Red). Minimization of the difference between λ max of the fluorophore and quencher maximized quenching efficiency.