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Highly Modular Structure and Ligand Binding by Conformational Capture in a Minimalistic Riboswitch
Author(s) -
DuchardtFerner Elke,
Weigand Julia E.,
Ohlenschläger Oliver,
Schmidtke Sina R.,
Suess Beatrix,
Wöhnert Jens
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001339
Subject(s) - riboswitch , ligand (biochemistry) , chemistry , nuclear magnetic resonance spectroscopy , stereochemistry , conformational change , rna , crystallography , biophysics , biology , biochemistry , receptor , gene , non coding rna
Be prepared : The structure of an synthetic neomycin riboswitch RNA (N1) is investigated by NMR spectroscopy. A largely disordered free structural ensemble also contains a compact conformation that resembles the ligand‐bound state thus suggesting a binding mechanism by conformational capture (see scheme, red=ligand).