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A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase
Author(s) -
Jang Hongje,
Kim YoungKwan,
Kwon HyunMi,
Yeo WoonSeok,
Kim DongEun,
Min DalHee
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001332
Subject(s) - helicase , duplex (building) , dna , fluorescence , graphene , primase , biophysics , double stranded , computational biology , chemistry , microbiology and biotechnology , nanotechnology , physics , biochemistry , biology , materials science , gene , polymerase chain reaction , rna , quantum mechanics , reverse transcriptase
Time to unwind : Graphene oxide (GO) enables the quantitative measurement of helicase‐dependent double‐stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescent‐dye‐labeled single‐stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.