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Fluorescent Cyclic Voltammetry of Immobilized Azurin: Direct Observation of Thermodynamic and Kinetic Heterogeneity
Author(s) -
Salverda Jante M.,
Patil Amol V.,
Mizzon Giulia,
Kuznetsova Sofya,
Zauner Gerhild,
Akkilic Namik,
Canters Gerard W.,
Davis Jason J.,
Heering Hendrik A.,
Aartsma Thijs J.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001298
Subject(s) - azurin , cyclic voltammetry , electron transfer , redox , chemistry , electrochemistry , copper protein , fluorescence , voltammetry , diffusion , analytical chemistry (journal) , electrode , dispersion (optics) , chemical physics , copper , inorganic chemistry , thermodynamics , organic chemistry , quantum mechanics , optics , physics
Variations in the formal electrochemical potential ( E 0 ) and electron‐transfer rates ( k 0 ) of the blue copper protein azurin have been directly observed. A new method, fluorescent cyclic voltammetry (FCV), was used to resolve the properties of 100–1000 proteins. On this scale, the presence of large variations in the values of both E 0 and k 0 could be established and several forms of heterogeneity were differentiated.