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Design and Folding of [Glu A4 (O β Thr B30 )]Insulin (“Ester Insulin”): A Minimal Proinsulin Surrogate that Can Be Chemically Converted into Human Insulin
Author(s) -
Sohma Youhei,
Hua QingXin,
Whittaker Jonathan,
Weiss Michael A.,
Kent Stephen B. H.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001151
Subject(s) - proinsulin , insulin , human insulin , saponification , yield (engineering) , disulfide bond , chemistry , biochemistry , medicine , materials science , metallurgy
Insulin folding : An ester‐linked polypeptide proinsulin surrogate folded efficiently with concomitant disulfide bond formation, and saponification gave native insulin having full biological activity. This strategy overcomes the low yield combination of individual insulin A and B chains, and provides a simple and effective approach to the total chemical synthesis of human insulin and its analogues.