Premium
Uniform Amplification of Phage with Different Growth Characteristics in Individual Compartments Consisting of Monodisperse Droplets
Author(s) -
Derda Ratmir,
Tang Sindy K. Y.,
Whitesides George M.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001143
Subject(s) - dispersity , phage display , phagemid , clone (java method) , competition (biology) , nanotechnology , biology , computational biology , chemistry , bacteriophage , genetics , materials science , dna , gene , polymer chemistry , escherichia coli , ecology , antibody
Save every clone! In phage display, clones displaying ligands that hinder growth of phage are lost in amplification. Competition of slowly (S) and rapidly (R) growing phage is mitigated in monodisperse emulsions generated by a simple microfluidic device. Separating R and S in ca. 10 7 droplets maintains R/S ratio throughout amplification. Competition‐free amplification of phage preserves ligands that are usually lost in phage display screen.