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Fused‐Gene Approach to Photoswitchable and Fluorescent Biliproteins
Author(s) -
Zhang Juan,
Wu XianJun,
Wang ZhiBin,
Chen Yu,
Wang Xing,
Zhou Ming,
Scheer Hugo,
Zhao KaiHong
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201001094
Subject(s) - fluorescence , chromophore , chemistry , heme , fluorescent protein , biochemistry , gene , green fluorescent protein , photochemistry , enzyme , physics , quantum mechanics
Lighting up and changing color : Genes coding for apoprotein and chromophore biosynthesis from heme were combined in a single fused construct, thereby providing access, in vivo, to biliproteins. Persistently fluorescent biliproteins, as well as red–green photoswitchable biliproteins derived from a cyanobacteriochrome that have well‐separated absorption and fluorescence maxima of the two states, were produced in E. coli.