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Label‐Free, Real‐Time Monitoring of Biomass Processing with Stimulated Raman Scattering Microscopy
Author(s) -
Saar Brian G.,
Zeng Yining,
Freudiger Christian W.,
Liu YuSan,
Himmel Michael E.,
Xie X. Sunney,
Ding ShiYou
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201000900
Subject(s) - lignin , raman scattering , cellulose , microscopy , biomass (ecology) , materials science , degradation (telecommunications) , resolution (logic) , raman spectroscopy , micrometer , scattering , nanotechnology , optics , chemistry , computer science , organic chemistry , artificial intelligence , telecommunications , physics , oceanography , geology
Label‐free “chemical movies” of lignin and cellulose in fresh plant tissue were recorded during a step in the breakdown of biomass to produce biofuels by using stimulated Raman scattering microscopy. The technique allows the degradation of lignin in the cell wall to be monitored with high sensitivity, sub‐micrometer spatial resolution, and high temporal resolution.