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In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids
Author(s) -
Lepthien Sandra,
Merkel Lars,
Budisa Nediljko
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201000439
Subject(s) - in vivo , amino acid , escherichia coli , synthetic biology , chemistry , biochemistry , protein biosynthesis , biology , computational biology , gene , genetics
Three in one : A new method has been developed for the simultaneous in vivo incorporation of two or three different synthetic amino acids in a protein in a single expression experiment using polyauxotrophic Escherichia coli strains. The picture shows the model protein ψ‐b* used and the synthetic amino acids incorporated.