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Inside Cover: Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High‐Molecular‐Weight Proteins (Angew. Chem. Int. Ed. 11/2010)
Author(s) -
Gans Pierre,
Hamelin Olivier,
Sounier Remy,
Ayala Isabel,
Durá M. Asunción,
Amero Carlos D.,
NoirclercSavoye Marjolaine,
Franzetti Bruno,
Plevin Michael J.,
Boisbouvier Jérôme
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201000347
Subject(s) - chemistry , valine , stereospecificity , protonation , nuclear magnetic resonance spectroscopy , isotopic labeling , stereochemistry , biomolecule , amino acid , organic chemistry , biochemistry , catalysis , ion
NMR spectroscopy of large protein assemblies necessitates targeted protonation of specific sites within a perdeuterated background. In their Communication on page 1958 ff., J. Boisbouvier et al. outline the use of an acetolactate precursor for the specific biosynthetic incorporation of 13 C 1 H 3 into the pro‐ S methyl groups of leucine and valine. This labeling strategy enhances spectral quality and provides an efficient basis for the application of solution‐state NMR techniques to complex biomolecules.