Premium
DNA‐Templated Synthesis of Trimethine Cyanine Dyes: A Versatile Fluorogenic Reaction for Sensing G‐Quadruplex Formation
Author(s) -
Meguellati Kamel,
Koripelly Girish,
Ladame Sylvain
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201000291
Subject(s) - cyanine , nucleic acid , chemistry , dna , g quadruplex , fluorescence , combinatorial chemistry , computational biology , computer science , nanotechnology , biochemistry , biology , materials science , physics , quantum mechanics
A healthy glow : Fluorogenic peptide nucleic acids (PNAs) functionalized with indoline derivatives are used to specifically sense G‐quadruplex formation. Upon hybridization of both PNAs to the single‐stranded flanking arms of quadruplex DNA (see scheme), the synthesis of a trimethine cyanine dye is templated. Dye formation can be detected by the appearance of a characteristic fluorescence signal.