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Inside Cover: Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains (Angew. Chem. Int. Ed. 15/2009)
Author(s) -
Wieland Markus,
Benz Armin,
Klauser Benedikt,
Hartig Jörg S.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200990073
Subject(s) - ribozyme , riboswitch , aptamer , mammalian cpeb3 ribozyme , rna , chemistry , cleavage (geology) , messenger rna , computational biology , vs ribozyme , biophysics , microbiology and biotechnology , biology , gene , biochemistry , non coding rna , paleontology , fracture (geology)
Fusions of a ribozyme and an aptamer of a natural riboswitch (thiamine pyrophosphate, TPP) are used to construct artificial thiamine‐dependent switches of gene expression. As J. S. Hartig et al. describe in their Communication on page 2715 ff. , insertion of these RNA elements into bacterial mRNAs allows translation of the message to be switched on or off. TPP triggers changes to the ribozyme‐mediated mRNA cleavage, resulting in liberation of the message for translational initiation.