A Nanogel for Ratiometric Fluorescent Sensing of Intracellular pH Values | Zendy

Peng Hongshang | Zendy; Stolwijk Judith A. | Zendy; Sun LiNing | Zendy; Wegener Joachim | Zendy; Wolfbeis Otto S. | Zendy

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A Nanogel for Ratiometric Fluorescent Sensing of Intracellular pH Values

Author(s) -

Peng Hongshang,

Stolwijk Judith A.,

Sun LiNing,

Wegener Joachim,

Wolfbeis Otto S.

Publication year - 2010

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200906926

Subject(s) - nanogel , fluorescence , nile red , coumarin , chemistry , biophysics , förster resonance energy transfer , intracellular ph , nile blue , intracellular , nanotechnology , photochemistry , materials science , biochemistry , organic chemistry , biology , drug delivery , optics , physics

A ratiometric fluorescent nanogel can sense pH over a range that is applicable to physiological studies. It can be easily prepared and made pH‐responsive by addition of a pH probe and a FRET system that utilizes the gel to hold dyes in close proximity (see picture; overlay of coumarin dye and Nile Red fluorescence in kidney cells).

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