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A Nanogel for Ratiometric Fluorescent Sensing of Intracellular pH Values
Author(s) -
Peng Hongshang,
Stolwijk Judith A.,
Sun LiNing,
Wegener Joachim,
Wolfbeis Otto S.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200906926
Subject(s) - nanogel , fluorescence , nile red , coumarin , chemistry , biophysics , förster resonance energy transfer , intracellular ph , nile blue , intracellular , nanotechnology , photochemistry , materials science , biochemistry , organic chemistry , biology , drug delivery , optics , physics
A ratiometric fluorescent nanogel can sense pH over a range that is applicable to physiological studies. It can be easily prepared and made pH‐responsive by addition of a pH probe and a FRET system that utilizes the gel to hold dyes in close proximity (see picture; overlay of coumarin dye and Nile Red fluorescence in kidney cells).