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High‐Resolution Studies of Uniformly 13 C, 15 N‐Labeled RNA by Solid‐State NMR Spectroscopy
Author(s) -
Cherepanov Alexey V.,
Glaubitz Clemens,
Schwalbe Harald
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200906885
Subject(s) - rna , nuclear magnetic resonance spectroscopy , spectroscopy , resolution (logic) , chemistry , solid state , high resolution , aqueous solution , analytical chemistry (journal) , crystallography , physics , stereochemistry , computer science , chromatography , biochemistry , quantum mechanics , remote sensing , artificial intelligence , gene , geology
Chilling out : Solid‐state 13 C NMR correlation spectroscopy was used to assign the signals of a uniformly labeled RNA hairpin in frozen aqueous solution. Conformational analysis shows that solutions of biologically relevant RNAs can freeze without significant changes in RNA structure and without critical loss of resolution and sensitivity in NMR experiments.