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Bisaryl Hydrazones as Exchangeable Biocompatible Linkers
Author(s) -
Dirksen Anouk,
Yegneswaran Subramanian,
Dawson Philip E.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200906756
Subject(s) - library science , national laboratory , chemistry , engineering , engineering physics , computer science
Catch and release : Stable bisaryl hydrazones undergo rapid exchange under mild conditions in the presence of a catalyst (see schematic illustration; b=biotin). This highly efficient hydrazone exchange is useful for the affinity purification of biotinylated proteins on (strept)avidin beads. The method enables the mild elution of label‐exchanged or chemically functionalized proteins.