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Detection of Single Enzyme Molecules inside Nanopores on the Basis of Chemiluminescence
Author(s) -
Kang Seong Ho,
Lee Seungah,
Yeung Edward S.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200906713
Subject(s) - chemiluminescence , nanopore , basis (linear algebra) , signal (programming language) , pixel , molecule , enzyme , chemistry , nanotechnology , optoelectronics , materials science , computer science , optics , physics , biochemistry , chromatography , organic chemistry , mathematics , geometry , programming language
Trapped, but not immobilized inside 50 nm membrane pores, luciferase was detected on the basis of chemiluminescence (see scheme) with an intensified‐charge‐coupled‐device (ICCD) camera. Light emission from the nanopores was concentrated onto individual pixels of the ICCD. Single‐molecule detection was possible by integration of the amplified signal over a prolonged enzyme reaction time.