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Small‐Molecule Affinity Ligands for Protein Purification: Combined Computational Enrichment and Automated In‐line Screening of an Optically Encoded Library
Author(s) -
Rasmussen Jakob E.,
Schiødt Christine B.,
Christensen Søren F.,
NørskovLauritsen Leif,
Meldal Morten,
St. Hilaire Phaedria M.,
Jensen Knud J.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200906602
Subject(s) - affinity chromatography , combinatorial chemistry , chemistry , computational biology , target protein , identification (biology) , small molecule , molecule , biochemistry , biology , organic chemistry , gene , enzyme , botany
Screening with codes : A versatile methodology for the development of small‐molecule affinity chromatography ligands for protein targets is presented. The combination of computational enrichment with automated screening and affinity mapping of an optically encoded combinatorial library allows identification of a novel set of ligands for the single‐step purification of human growth hormone (see picture).