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An Efficient Protocol for NMR‐Spectroscopy‐Based Structure Determination of Protein Complexes in Solution
Author(s) -
Simon Bernd,
Madl Tobias,
Mackereth Cameron D.,
Nilges Michael,
Sattler Michael
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200906147
Subject(s) - nuclear magnetic resonance spectroscopy , spectroscopy , domain (mathematical analysis) , protocol (science) , resolution (logic) , high resolution , computer science , chemistry , information retrieval , crystallography , mathematics , physics , artificial intelligence , stereochemistry , medicine , geography , remote sensing , mathematical analysis , quantum mechanics , alternative medicine , pathology
Putting the pieces together : An efficient, generally applicable approach for structural analysis of protein complexes and multidomain proteins in solution based on NMR spectroscopy is presented. Starting from available high‐resolution structures of individual domains or subunits, the overall domain arrangement is calculated from NMR spectroscopy data that can be obtained for high‐molecular‐weight complexes.