Premium
Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High‐Molecular‐Weight Proteins
Author(s) -
Gans Pierre,
Hamelin Olivier,
Sounier Remy,
Ayala Isabel,
Durá M. Asunción,
Amero Carlos D.,
NoirclercSavoye Marjolaine,
Franzetti Bruno,
Plevin Michael J.,
Boisbouvier Jérôme
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200905660
Subject(s) - stereospecificity , isotopomers , chemistry , valine , isotopic labeling , leucine , stereochemistry , nuclear magnetic resonance spectroscopy , amino acid , molecule , organic chemistry , biochemistry , catalysis
Sometimes less is more : [ 13 C 1 H 3 ]methyl isotopomers can be biosynthetically incorporated specifically into the pro‐ S methyl groups of leucine and valine residues in large protein assemblies within a perdeuterated background by using an acetolactate precursor. This stereospecific labeling strategy considerably enhances NMR spectra for large protein assemblies.