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Phosphorylation‐Dependent Kinase–Substrate Cross‐Linking
Author(s) -
Suwal Sujit,
Pflum Mary Kay H.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200905244
Subject(s) - kinase , phosphorylation , substrate level phosphorylation , substrate (aquarium) , effector , information retrieval , computer science , computational biology , chemistry , biochemistry , microbiology and biotechnology , biology , ecology
Pinning down kinase substrates : The identification of substrates of a particular kinase is fundamental to the elucidation of cell‐signaling cascades. This problem has now been addressed by the title approach involving kinase‐catalyzed labeling coupled with photo‐cross‐linking (see scheme). When coupled with mass spectrometry analysis, this strategy can be used to determine the sites of phosphorylation as well as the effector kinase. ADP=adenosine 5′‐diphosphate.