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Expanded Genetic Alphabets in the Polymerase Chain Reaction
Author(s) -
Yang Zunyi,
Chen Fei,
Chamberlin Stephen G.,
Benner Steven A.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200905173
Subject(s) - polymerase chain reaction , polymerase chain reaction optimization , computational biology , genetics , biology , nested polymerase chain reaction , gene
Cleaning up polymerase chain reactions : DNA polymerases are found that copy two additional nucleotide letters (Z and P) in an expanded DNA alphabet to support six‐letter polymerase chain reactions (PCR). Incorporated into external primers in a threefold multiplexed PCR, primers containing Z and P gave much cleaner results than standard multiplexed PCR.
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