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Detection of Proteins in Serum by Micromagnetic Aptamer PCR (MAP) Technology
Author(s) -
Csordas Andrew,
Gerdon Aren E.,
Adams Jonathan D.,
Qian Jiangrong,
Oh Seung Soo,
Xiao Yi,
Soh H. Tom
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200904846
Subject(s) - aptamer , detection limit , fetal bovine serum , microfluidics , chromatography , range (aeronautics) , microbiology and biotechnology , analytical chemistry (journal) , biology , computational biology , chemistry , materials science , nanotechnology , genetics , in vitro , composite material
On the MAP : The title technique combines microfluidic magnetic sample preparation with highly specific aptamer‐based molecular recognition and quantitative PCR. Using platelet‐derived growth factor as a model, a f M detection limit was obtained in fetal bovine serum with a dynamic range that spanned five orders of magnitude.
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