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Single‐Molecule DNA Biosensors for Protein and Ligand Detection
Author(s) -
Lymperopoulos Konstantinos,
Crawford Robert,
Torella Joseph P.,
Heilemann Mike,
Hwang Ling Chin,
Holden Seamus J.,
Kapanidis Achillefs N.
Publication year - 2010
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200904597
Subject(s) - dna , biosensor , molecule , nucleotide , chemistry , fluorescence , transcription factor , computational biology , biophysics , small molecule , ligand (biochemistry) , gene , biology , microbiology and biotechnology , biochemistry , receptor , physics , organic chemistry , quantum mechanics
Transcription factors (TFs) control gene expression and are promising biomarkers for diseases. In a new, robust, and sensitive single‐molecule fluorescence assay TFs and associated small molecules (such as nucleotides and sugars) are detected based on DNA coincidence of the two halves of the binding site (see picture; S=stoichiometry). The assay is compatible with measurements in solution, on solid supports, and even in complex biological samples.