Premium
A Pyrrolysine Analogue for Site‐Specific Protein Ubiquitination
Author(s) -
Li Xin,
Fekner Tomasz,
Ottesen Jennifer J.,
Chan Michael K.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200904472
Subject(s) - ubiquitin , residue (chemistry) , lysine , native chemical ligation , polypeptide chain , chemistry , side chain , stereochemistry , biochemistry , computational biology , amino acid , biology , chemical synthesis , in vitro , organic chemistry , gene , polymer
The art of stitching proteins : D ‐Cys‐ε‐Lys and its diastereomer L ‐Cys‐ε‐Lys read through the UAG codon (see scheme). As the resulting proteins can participate in native chemical ligation (NCL), this process provides a means to prepare proteins chemoselectively modified (e.g. ubiquitinated) through a peptidic side chain located at the ε position of a rationally selected lysine residue.