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Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Proteins Containing Nonnatural Amino Acids
Author(s) -
Guo Jiantao,
Melançon Charles E.,
Lee Hyun Soo,
Groff Dan,
Schultz Peter G.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200904035
Subject(s) - transfer rna , amino acid , protein biosynthesis , biochemistry , computational biology , chemistry , in vitro , biology , gene , rna
Applied evolution : Regions of the M. jannaschii tyrosyl tRNA CUA thought to interact with elongation factor Tu were randomized, and the resulting tRNA libraries were subjected to in vitro evolution. The tRNAs identified resulted in significantly improved yields of proteins containing nonnatural amino acids. In some cases, the degree of improvement varied in an amino acid dependent manner.