Optical Thermophoresis for Quantifying the Buffer Dependence of Aptamer Binding | Zendy

Baaske Philipp | Zendy; Wienken Christoph J. | Zendy; Reineck Philipp | Zendy; Duhr Stefan | Zendy; Braun Dieter | Zendy

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Optical Thermophoresis for Quantifying the Buffer Dependence of Aptamer Binding

Author(s) -

Baaske Philipp,

Wienken Christoph J.,

Reineck Philipp,

Duhr Stefan,

Braun Dieter

Publication year - 2010

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200903998

Subject(s) - aptamer , thermophoresis , microscale thermophoresis , diagram , buffer (optical fiber) , sample (material) , computer science , molecule , biological system , chemistry , nanotechnology , information retrieval , computational biology , chromatography , materials science , biology , biochemistry , genetics , database , nanoparticle , telecommunications , nanofluid , organic chemistry

Some like it hot : A robust and fast method for characterizing aptamers relies on the distinct thermophoretic movements of molecules in microscopic temperature gradients (see diagram). The binding properties of proteins and even small molecules can be measured within seconds, and less than 1 μL of sample is required. Notably, the technique works well in complex liquids such as human serum.

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