A Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins | Zendy

Ren Hongjun | Zendy; Xiao Fei | Zendy; Zhan Ke | Zendy; Kim YoungPil | Zendy; Xie Hexin | Zendy; Xia Zuyong | Zendy; Rao Jianghong | Zendy

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A Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins

Author(s) -

Ren Hongjun,

Xiao Fei,

Zhan Ke,

Kim YoungPil,

Xie Hexin,

Xia Zuyong,

Rao Jianghong

Publication year - 2009

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200903627

Subject(s) - cysteine , rhodamine , chemistry , residue (chemistry) , in vitro , biochemistry , fluorescence , amino acid residue , condensation reaction , combinatorial chemistry , molecule , biophysics , peptide sequence , organic chemistry , biology , catalysis , enzyme , physics , quantum mechanics , gene

Going live : A protein‐labeling method based on the use of a single amino acid tag—an N‐terminal cysteine residue—and small‐molecule probes containing a cyanobenzothiazole (CBT) unit has been used for the specific fluorescence labeling of proteins in vitro and at the surface of live cells (see scheme). This simple ligation reaction proceeds with a high degree of specificity under physiological conditions. Rd: a rhodamine dye; TEV: tobacco etch virus.

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